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samples human t  (DSMZ)


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    Structured Review

    DSMZ samples human t
    Samples Human T, supplied by DSMZ, used in various techniques. Bioz Stars score: 94/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/samples human t/product/DSMZ
    Average 94 stars, based on 26 article reviews
    samples human t - by Bioz Stars, 2026-06
    94/100 stars

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    DSMZ samples human t
    Fig. 3. DECTIN-1 expression and immunophenotyping of the kindred. (A) Flow cytometric plots showing gating strategy for detecting DECTIN-1 expression on monocytes (CD14+) from PBMCs. (B) Quantification of the frequency of DECTIN-1+ cells among CD14+ monocytes and DECTIN-1 MFI in CD14+ monocytes from PBMCs in the proband (red), a healthy control (dark gray), and an FMO (light gray). (C) Flow cytometric plots, and (D) quantification of Tregs (CD25+FOXP3+) from the patient and healthy controls. (E to H) Representative flow cytometric profiles and quantified frequencies of CXCR5−/CXCR5+ TH1 (CXCR3+CCR6−) and TH2 <t>(CXCR3−CCR6−)</t> <t>CD4+</t> cells and CXCR5+ (TFH) effector (CCR7+) and memory (PD1+) cells from PBMCs of the kindred and healthy blood donors. Each dot is representative of a separate <t>PBMC</t> donor. In (F to J), the colors are the same as those in the legend for (B) and (D).
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    USP2 protein expression levels were reduced <t>in</t> <t>HCC-T</t> tissues from HCC patients. (A) Representative total USP2 protein staining with goat anti-human USP2 IgG in the tissues of human normal liver (n=76), HCC-NT (n=38) and HCC-T (n=115) by IHC. As negative controls, human normal liver tissues (n=6) were also stained with goat pre-immune serum. (B) Quantification of the USP2 protein levels were performed with ImageJ. (C) Representative USP2 protein staining in four paired HCC-T and HCC-NT tissues (n=10) by IHC. (D) USP2 protein levels in ten paired HCC-NT and HCC-T tissues as groups as well as (E) individual pairs. **P<0.01 and ***P<0.001 in students’ t-test for pair-wise comparison or one-way ANOVA, followed by Tukey post-hoc test for multiple comparisons.
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    USP2 protein expression levels were reduced <t>in</t> <t>HCC-T</t> tissues from HCC patients. (A) Representative total USP2 protein staining with goat anti-human USP2 IgG in the tissues of human normal liver (n=76), HCC-NT (n=38) and HCC-T (n=115) by IHC. As negative controls, human normal liver tissues (n=6) were also stained with goat pre-immune serum. (B) Quantification of the USP2 protein levels were performed with ImageJ. (C) Representative USP2 protein staining in four paired HCC-T and HCC-NT tissues (n=10) by IHC. (D) USP2 protein levels in ten paired HCC-NT and HCC-T tissues as groups as well as (E) individual pairs. **P<0.01 and ***P<0.001 in students’ t-test for pair-wise comparison or one-way ANOVA, followed by Tukey post-hoc test for multiple comparisons.
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    Image Search Results


    Fig. 3. DECTIN-1 expression and immunophenotyping of the kindred. (A) Flow cytometric plots showing gating strategy for detecting DECTIN-1 expression on monocytes (CD14+) from PBMCs. (B) Quantification of the frequency of DECTIN-1+ cells among CD14+ monocytes and DECTIN-1 MFI in CD14+ monocytes from PBMCs in the proband (red), a healthy control (dark gray), and an FMO (light gray). (C) Flow cytometric plots, and (D) quantification of Tregs (CD25+FOXP3+) from the patient and healthy controls. (E to H) Representative flow cytometric profiles and quantified frequencies of CXCR5−/CXCR5+ TH1 (CXCR3+CCR6−) and TH2 (CXCR3−CCR6−) CD4+ cells and CXCR5+ (TFH) effector (CCR7+) and memory (PD1+) cells from PBMCs of the kindred and healthy blood donors. Each dot is representative of a separate PBMC donor. In (F to J), the colors are the same as those in the legend for (B) and (D).

    Journal: Science advances

    Article Title: DECTIN-1: A modifier protein in CTLA-4 haploinsufficiency.

    doi: 10.1126/sciadv.adi9566

    Figure Lengend Snippet: Fig. 3. DECTIN-1 expression and immunophenotyping of the kindred. (A) Flow cytometric plots showing gating strategy for detecting DECTIN-1 expression on monocytes (CD14+) from PBMCs. (B) Quantification of the frequency of DECTIN-1+ cells among CD14+ monocytes and DECTIN-1 MFI in CD14+ monocytes from PBMCs in the proband (red), a healthy control (dark gray), and an FMO (light gray). (C) Flow cytometric plots, and (D) quantification of Tregs (CD25+FOXP3+) from the patient and healthy controls. (E to H) Representative flow cytometric profiles and quantified frequencies of CXCR5−/CXCR5+ TH1 (CXCR3+CCR6−) and TH2 (CXCR3−CCR6−) CD4+ cells and CXCR5+ (TFH) effector (CCR7+) and memory (PD1+) cells from PBMCs of the kindred and healthy blood donors. Each dot is representative of a separate PBMC donor. In (F to J), the colors are the same as those in the legend for (B) and (D).

    Article Snippet: Human Treg differentiation and suppression assays Naïve CD4 T cells were magnetically sorted from frozen PBMC samples (Naïve CD4+ T cell isolation kit: 130-094-131, Miltenyi Biotech), and cultured with recombinant TGF-β (5 ng/ml), IL-2 (1 mg/ml), and anti-CD3/anti-CD28 activation beads (T cell activation kit: 130–091-441, Miltenyi Biotech), in the absence or presence of depleted zymosan (100 μg/ml) stimulation.

    Techniques: Expressing, Control

    USP2 protein expression levels were reduced in HCC-T tissues from HCC patients. (A) Representative total USP2 protein staining with goat anti-human USP2 IgG in the tissues of human normal liver (n=76), HCC-NT (n=38) and HCC-T (n=115) by IHC. As negative controls, human normal liver tissues (n=6) were also stained with goat pre-immune serum. (B) Quantification of the USP2 protein levels were performed with ImageJ. (C) Representative USP2 protein staining in four paired HCC-T and HCC-NT tissues (n=10) by IHC. (D) USP2 protein levels in ten paired HCC-NT and HCC-T tissues as groups as well as (E) individual pairs. **P<0.01 and ***P<0.001 in students’ t-test for pair-wise comparison or one-way ANOVA, followed by Tukey post-hoc test for multiple comparisons.

    Journal: American Journal of Cancer Research

    Article Title: Dysregulation and activities of ubiquitin specific peptidase 2b in the pathogenesis of hepatocellular carcinoma

    doi:

    Figure Lengend Snippet: USP2 protein expression levels were reduced in HCC-T tissues from HCC patients. (A) Representative total USP2 protein staining with goat anti-human USP2 IgG in the tissues of human normal liver (n=76), HCC-NT (n=38) and HCC-T (n=115) by IHC. As negative controls, human normal liver tissues (n=6) were also stained with goat pre-immune serum. (B) Quantification of the USP2 protein levels were performed with ImageJ. (C) Representative USP2 protein staining in four paired HCC-T and HCC-NT tissues (n=10) by IHC. (D) USP2 protein levels in ten paired HCC-NT and HCC-T tissues as groups as well as (E) individual pairs. **P<0.01 and ***P<0.001 in students’ t-test for pair-wise comparison or one-way ANOVA, followed by Tukey post-hoc test for multiple comparisons.

    Article Snippet: Human liver samples Human liver tissue microarray slides including a total of 76 normal, 38 HCC-NT and 115 HCC-T samples with 10 pairs of HCC-NT and HCC-T samples were purchased from US Biomax (Cat# BC03119b-SC2, LV241a-D024, LVN241-T202 and LVN801-H139).

    Techniques: Expressing, Staining

    USP2 mRNA expression levels were decreased in HCC-T tissues from HCC patients. (A) The expression levels of USP2 mRNA in human normal liver (n=12), HCC-NT (n=8) and HCC-T (n=21) detected by real-time PCR. (B) The expression levels of USP2 mRNA in paired HCC-NT and HCC-T tissues as groups as well as (C) individual pairs (n=8). *P<0.05 and **P<0.01 in students’ t-test for pair-wise comparison or one-way ANOVA, followed by Tukey post-hoc test for multiple comparisons.

    Journal: American Journal of Cancer Research

    Article Title: Dysregulation and activities of ubiquitin specific peptidase 2b in the pathogenesis of hepatocellular carcinoma

    doi:

    Figure Lengend Snippet: USP2 mRNA expression levels were decreased in HCC-T tissues from HCC patients. (A) The expression levels of USP2 mRNA in human normal liver (n=12), HCC-NT (n=8) and HCC-T (n=21) detected by real-time PCR. (B) The expression levels of USP2 mRNA in paired HCC-NT and HCC-T tissues as groups as well as (C) individual pairs (n=8). *P<0.05 and **P<0.01 in students’ t-test for pair-wise comparison or one-way ANOVA, followed by Tukey post-hoc test for multiple comparisons.

    Article Snippet: Human liver samples Human liver tissue microarray slides including a total of 76 normal, 38 HCC-NT and 115 HCC-T samples with 10 pairs of HCC-NT and HCC-T samples were purchased from US Biomax (Cat# BC03119b-SC2, LV241a-D024, LVN241-T202 and LVN801-H139).

    Techniques: Expressing, Real-time Polymerase Chain Reaction

    Dysregulation of Usp2 mRNA expression in HCC-T tissues from HCC mice. A. The expression levels of Usp2 mRNA in mouse normal liver (n=16), HCC-NT (n=15) and HCC-T (n=15) samples. B. The expression levels of USP2 mRNA in paired HCC-T and HCC-NT tissues. **P<0.01 and ***P<0.001 in students’ t-test for pair-wise comparison or one-way ANOVA, followed by Tukey post-hoc test for multiple comparisons.

    Journal: American Journal of Cancer Research

    Article Title: Dysregulation and activities of ubiquitin specific peptidase 2b in the pathogenesis of hepatocellular carcinoma

    doi:

    Figure Lengend Snippet: Dysregulation of Usp2 mRNA expression in HCC-T tissues from HCC mice. A. The expression levels of Usp2 mRNA in mouse normal liver (n=16), HCC-NT (n=15) and HCC-T (n=15) samples. B. The expression levels of USP2 mRNA in paired HCC-T and HCC-NT tissues. **P<0.01 and ***P<0.001 in students’ t-test for pair-wise comparison or one-way ANOVA, followed by Tukey post-hoc test for multiple comparisons.

    Article Snippet: Human liver samples Human liver tissue microarray slides including a total of 76 normal, 38 HCC-NT and 115 HCC-T samples with 10 pairs of HCC-NT and HCC-T samples were purchased from US Biomax (Cat# BC03119b-SC2, LV241a-D024, LVN241-T202 and LVN801-H139).

    Techniques: Expressing

    USP2b mRNA expression levels were markedly reduced in HCC-T tissues from HCC patients. (A) The expression levels of USP2 mRNA in human normal liver (n=12), HCC-NT (n=8) and HCC-T (n=21) detected by real-time PCR. (B) USP2b protein detected by Western blot in normal liver (n=8), HCC-NT (n=8) and HCC-T tissues (n=8). (C) Quantification of the USP2b protein levels in (B) by ImageJ. (D) The expression levels of USP2 mRNA in paired HCC-NT and HCC-T tissues as groups as well as (E) individual pairs (n=8). ***P<0.001 in students’ t-test for pair-wise comparison or one-way ANOVA, followed by Tukey post-hoc test for multiple comparisons.

    Journal: American Journal of Cancer Research

    Article Title: Dysregulation and activities of ubiquitin specific peptidase 2b in the pathogenesis of hepatocellular carcinoma

    doi:

    Figure Lengend Snippet: USP2b mRNA expression levels were markedly reduced in HCC-T tissues from HCC patients. (A) The expression levels of USP2 mRNA in human normal liver (n=12), HCC-NT (n=8) and HCC-T (n=21) detected by real-time PCR. (B) USP2b protein detected by Western blot in normal liver (n=8), HCC-NT (n=8) and HCC-T tissues (n=8). (C) Quantification of the USP2b protein levels in (B) by ImageJ. (D) The expression levels of USP2 mRNA in paired HCC-NT and HCC-T tissues as groups as well as (E) individual pairs (n=8). ***P<0.001 in students’ t-test for pair-wise comparison or one-way ANOVA, followed by Tukey post-hoc test for multiple comparisons.

    Article Snippet: Human liver samples Human liver tissue microarray slides including a total of 76 normal, 38 HCC-NT and 115 HCC-T samples with 10 pairs of HCC-NT and HCC-T samples were purchased from US Biomax (Cat# BC03119b-SC2, LV241a-D024, LVN241-T202 and LVN801-H139).

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Western Blot

    Usp2b mRNA expression levels were significantly deceased in HCC-T tissues from HCC mice. (A) The expression levels of Usp2b mRNA in mouse normal liver (n=16), HCC-NT (n=26) and HCC-T (n=26) samples. (B) The expression levels of Usp2b mRNA in paired HCC-T and HCC-NT tissues (n=26). (C) Representative Western blots of Usp2b protein in normal liver (n=18), HCC-NT (n=18) and HCC-T tissues (n=17). (D) Quantification of Usp2b protein in (C) by ImageJ. *P<0.05, **P<0.01 and ***P<0.001 in one-way ANOVA, followed by Tukey post-hoc test for multiple comparisons.

    Journal: American Journal of Cancer Research

    Article Title: Dysregulation and activities of ubiquitin specific peptidase 2b in the pathogenesis of hepatocellular carcinoma

    doi:

    Figure Lengend Snippet: Usp2b mRNA expression levels were significantly deceased in HCC-T tissues from HCC mice. (A) The expression levels of Usp2b mRNA in mouse normal liver (n=16), HCC-NT (n=26) and HCC-T (n=26) samples. (B) The expression levels of Usp2b mRNA in paired HCC-T and HCC-NT tissues (n=26). (C) Representative Western blots of Usp2b protein in normal liver (n=18), HCC-NT (n=18) and HCC-T tissues (n=17). (D) Quantification of Usp2b protein in (C) by ImageJ. *P<0.05, **P<0.01 and ***P<0.001 in one-way ANOVA, followed by Tukey post-hoc test for multiple comparisons.

    Article Snippet: Human liver samples Human liver tissue microarray slides including a total of 76 normal, 38 HCC-NT and 115 HCC-T samples with 10 pairs of HCC-NT and HCC-T samples were purchased from US Biomax (Cat# BC03119b-SC2, LV241a-D024, LVN241-T202 and LVN801-H139).

    Techniques: Expressing, Western Blot