Journal: Science advances
Article Title: DECTIN-1: A modifier protein in CTLA-4 haploinsufficiency.
doi: 10.1126/sciadv.adi9566
Figure Lengend Snippet: Fig. 3. DECTIN-1 expression and immunophenotyping of the kindred. (A) Flow cytometric plots showing gating strategy for detecting DECTIN-1 expression on monocytes (CD14+) from PBMCs. (B) Quantification of the frequency of DECTIN-1+ cells among CD14+ monocytes and DECTIN-1 MFI in CD14+ monocytes from PBMCs in the proband (red), a healthy control (dark gray), and an FMO (light gray). (C) Flow cytometric plots, and (D) quantification of Tregs (CD25+FOXP3+) from the patient and healthy controls. (E to H) Representative flow cytometric profiles and quantified frequencies of CXCR5−/CXCR5+ TH1 (CXCR3+CCR6−) and TH2 (CXCR3−CCR6−) CD4+ cells and CXCR5+ (TFH) effector (CCR7+) and memory (PD1+) cells from PBMCs of the kindred and healthy blood donors. Each dot is representative of a separate PBMC donor. In (F to J), the colors are the same as those in the legend for (B) and (D).
Article Snippet: Human Treg differentiation and suppression assays Naïve CD4 T cells were magnetically sorted from frozen PBMC samples (Naïve CD4+ T cell isolation kit: 130-094-131, Miltenyi Biotech), and cultured with recombinant TGF-β (5 ng/ml), IL-2 (1 mg/ml), and anti-CD3/anti-CD28 activation beads (T cell activation kit: 130–091-441, Miltenyi Biotech), in the absence or presence of depleted zymosan (100 μg/ml) stimulation.
Techniques: Expressing, Control